Fig. 3
One-dimensional 1H NMR spectra of AVEBE MD20 and its products following incubation with GTFB preparations. The spectra were recorded in D2O at 300 K after the incubation of 5 % AVEBE MD20 (a) with different GTFB preparations which have equal hydrolysis activity (25.0 μg/ml soluble GTFB, b, 38.7 μg/ml refolded GTFB, c and 249.4 μg/ml 25 °C ncIB GTFB, d) after 72 h at 37 °C and pH 4.7. Rα/β represent the reducing –(1 → 4)-D-Glcp units and Gα/β represent the D-Glcp units