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Fig. 2 | BMC Biotechnology

Fig. 2

From: A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis

Fig. 2

Optimization of the SLiCE reaction. The insert glucose-6-phosphate dehydrogenase 1 (G6PDH1) DNA fragments were amplified using 19-bp overlap primers. The linearized vectors were prepared by PCR amplification. The SLiCE reaction (from JM109 strain of E. coli) was performed at 37 °C. Each value is the mean ± standard deviation of three independent experiments. a Time course of the SLiCE reaction. The insert G6PDH1 DNA fragments and linearized vector were mixed in a molar ratio of 2:1. b The effect of the molar ratio of insert (G6PDH1) DNA fragment to vector on the number of colonies formed. The SLiCE reaction was performed for 10 min

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BMC Biotechnology

ISSN: 1472-6750

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