Fig. 9
From: Active immunization with Tocilizumab mimotopes induces specific immune responses
Western blot and immunofluorescence staining with mimotope-induced antibodies for IL-6R recognition in FLS-RA. a Fibroblast-like synoviocytes lysates from three patients with RA were loaded with 40 μg/per lane. IL-6R expressed in FLS-RA detection using Tocilizumab (lane 1) and the sera from mice immunized with the 4A220-KLH conjugate (lane 2), the 4A124-KLH conjugate (lane 3), 4A125-KLH conjugate (lane 4), 4A126-KLH conjugate (lane 5), the control peptide-KLH conjugate (lane 6), or the carrier KLH alone (lane 7). Sera were diluted with 1:1000. b Represent image of IL-6R recognition in FLS-RA. FLS-RA cells were incubated with Tocilizumab and pooled serum followed by FITC-labeled secondary antibodies where the nuclei were stained with DAPI. (a) sera from mice immunized with 4A220-KLH, (b) sera from mice immunized with 4A124-KLH, (c) sera from mice immunized with 4A125-KLH, (d) sera from mice immunized with 4A126-KLH, (e) Tocilizumab, (f) sera from mice immunized with the control peptide-KLH, (g) sera from mice immunized with KLH alone. The results showed that the antibodies in the sera of the mice immunized with mimotopes could recognize IL-6R expressed in FLS-RA