Fig. 1
From: Active immunization with Tocilizumab mimotopes induces specific immune responses
Four phage mimics were isolated and specifically recognized by Tocilizumab. Four rounds of biopanning were performed to identify the phage mimics. a The phage titer increased from 1.5 × 105 pfu/ml (first round) to 2.7 × 105 pfu/ml (second round) to 6.0 × 105 (third round) finally to 6.8 × 107 pfu/ml (fourth round). b Specificity ELISA was then performed to observe whether the whole fourth amplified phage could be specifically recognized by the Tocilizumab. The results showed the whole fourth amplified phage could be well recognized by both Tocilizumab and isotype control. Then 37 phage clones was selected and sequenced, and 7 different insert sequences were observed. Then we performed experiments to observe whether the seven different peptides displayed could be specifically recognized by the Tocilizumab. The results showed that four mimotope candidates were specifically recognized by Tocilizumab but not by the isotype-matched control antibody when 109 pfu (c) or 107 pfu (d) of the isolated phage particles were used (mean ± SD, *, P < 0.05; **, P < 0.005)