Alpha-l-Fucosidase Isoenzyme iso2 from Paenibacillus thiaminolyticus

BMC Biotechnology

Table 2 Results of transfucosylation reactions catalyzed by α-l-fucosidase iso2 from P. thiaminolyticus

Acceptor	Transglycosylation products	Acceptor	Transglycosylation products
pNPα-L-Fuc	+	methanol	+/*
pNPα-D-Gal	+	ethanol	+/*
pNPα-D-Glc	+	1-propanol	-
pNPα-D-Man	+	2-propanol	-
D-glucose	+	butanol	-
D-galactose	+	pentanol	-
D-fructose	+	1-octanol	-
D-mannose	+	Boc-L-Ser-OMe	-
D-glucosamine	+	Boc-L-Thr-OMe	-
N-acetyl-D-glucosamine	+/*
D-maltose	+

pNPα-l-Fuc served as a donor of α-l-fucosyl moiety in all cases
The symbol + indicates that the enzyme was able to use the acceptor for α- l -fucosyl transfer
The symbol - indicates that no transglycosylation product was detected by TLC
The symbol * indicates that the transglycosylation product was confirmed by mass spectrometry

ISSN: 1472-6750