Table 1 Comparison of kinetic parameters of α-l-fucosidase isoenzymes from P. thiaminolyticus using chromogenic substrate pNPα-l-Fuc
From: Alpha-l-Fucosidase Isoenzyme iso2 from Paenibacillus thiaminolyticus
α-L-fucosidase | ||
|---|---|---|
iso1 | iso2 | |
Km | 0.44 ± 0.02 mmol/L | 0.52 ± 0.05 mmol/L |
Ks | 83 ± 8 mmol/L | 79 ± 20 mmol/L |
kcat | 58.7 ± 0.8 s-1 | 111 ± 3 s-1 |
kcat/Km | 133 ± 7 (mmol/L)-1 s-1 | 213 ± 30 (mmol/L)-1 s-1 |