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Fig. 9 | BMC Biotechnology

Fig. 9

From: Does size matter? Separations on guard columns for fast sample analysis applied to bioenergy research

Fig. 9

Chromatogram of (a) the supernatant of Escherichia coli strain DH1 fermentation showing n-butanol (peak 1) elution at 0.6 ml/min flow rate, (b) the hydrolysate 3 showing 5-HMF (peak 2) and furfural (peak 3) elution at 0.6 ml/min flow rate, (c) a mixture of levulinic acid, 2,5-hexanedione, 5-HMF, 2,5-dimethyltetrahydrofuran, furfural, methylfuran, 5-methylfurfural and 2,5-dimethylfuran at 0.6 ml/min flow rate, (d) a standard of n-hexanol (peak 4) at 1.0 ml/min flow rate, using the 30 mm guard column. HPLC parameters: column temperature 55 °C, mobile phase 5 mM sulfuric acid, flow rate 0.6 mL/min (1.0 mL/min for n-hexanol), refractive index detection

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