Fig. 7
Chromatogram of the supernatant from the enzymatic digestion of “pretreated Miscanthus biomass” showing citric acid (peak 1), glucose (peak 2), sorbitol (peak 3) and acetic acid (peak 4) elution after (a) 0 h and 24 h digestion in citrate buffer analyzed with the 50 mm guard column, (b) 0 h and 24 h digestion in citrate buffer analyzed with the 300 mm column, (c) 24 h digestion in acetate buffer analyzed with the 50 mm guard column; and enzymatically released glucan concentration profile of the supernatant from the enzymatic digestion of “pretreated Miscanthus biomass”between 0 h and 72 h using (d) citrate buffer (e) acetate buffer analyzed with the 50 mm guard column and 300 mm column. HPLC parameters: column temperature 55 °C, mobile phase 5 mM sulfuric acid, flow rate 1.0 mL/min (0.6 mL/min for 300 mm column), refractive index detection. Error bars represent +/− 1 standard deviation (n = 3)