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Fig. 3 | BMC Biotechnology

Fig. 3

From: Does size matter? Separations on guard columns for fast sample analysis applied to bioenergy research

Fig. 3

Representative chromatogram of the supernatant from Escherichia coli strain sFAB5692 fermentation analyzed with (a) the 50 mm guard column showing iso-butanol (peak 1) elution, and (b) iso-butanol concentration profile of the supernatant from E. coli strains sFAB5441 and sFAB5692 fermentation between 24 h and 192 h analyzed with the 50 mm guard column and 300 mm column. HPLC parameters: column temperature 55 °C, mobile phase 5 mM sulfuric acid, flow rate 1.0 mL/min (0.6 mL/min for 300 mm column), refractive index detection

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BMC Biotechnology

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