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Table 2 The kinetic constants of allergen-specific Fab fragments measured by BIAcore

From: Isolation of Mal d 1 and Api g 1 - specific recombinant antibodies from mouse IgG Fab fragment libraries – Mal d 1-specific antibody exhibits cross-reactivity against Bet v 1

Allergen

Fab

ka (1/Ms)

kd (1/s)

KD (M)

rMal d 1

MA1

7.340 × 105 ± 1.3 × 103

0.002427 ± 2.1 × 10−6

3.306 × 10−9

rMal d 1

MB3

2.990 × 105 ± 1.6 × 103

0.005890 ± 2.1 × 10−5

1.970 × 10−8

rApi g 1

AH7

8.056 × 104 ± 1.6 × 103

0.04323 ± 7.6 × 10−4

5.366 × 10−7

rApi g 1

AA11

8.533 × 104 ± 5.1 × 102

0.05931 ± 2.1 × 10−4

6.950 × 10−7

  1. The association (ka) and dissociation (kd) rate constants and affinity constant (KD) have been measured for the Fab fragments. The values present the averages (±SD) obtained with seven different Fab fragment concentrations.
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BMC Biotechnology

ISSN: 1472-6750

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