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Fig. 8 | BMC Biotechnology

Fig. 8

From: Isolation of Mal d 1 and Api g 1 - specific recombinant antibodies from mouse IgG Fab fragment libraries – Mal d 1-specific antibody exhibits cross-reactivity against Bet v 1

Fig. 8

Sandwich ELISA. The sandwich ELISA principle (a) and result (b) are shown to one sandwich couple per allergen. The immobilised antibody is the purified Fab fragment (MB3 or AH7 Fab fragment) whereas the detection antibody is displayed on the tip of the phage particle (MA1 or AA11 Fab fragment displaying phage). The front view of Bet v 1 (left, PDB:1BV1, [40]) and Api g 1 (right, PDB:2BK0, [14]) crystal structures are from Protein Data Bank. Since Mal d 1 crystal structure is not accessible, the structure of its homologue Bet v 1 was used here as a model of Mal d 1 allergen

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BMC Biotechnology

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