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Fig. 7 | BMC Biotechnology

Fig. 7

From: Isolation of Mal d 1 and Api g 1 - specific recombinant antibodies from mouse IgG Fab fragment libraries – Mal d 1-specific antibody exhibits cross-reactivity against Bet v 1

Fig. 7

Binding of the MA1 Fab fragment to rBet v 1 measured by immunoprecipitation assay. A silver-stained SDS-PAGE gel with low molecular weight marker (lane 1), only beads (lane 2), beads incubated with Fab fragment (lane 3), beads incubated with Fab fragment and rBet v 1 (Biomay, lane 4), beads incubated with Fab fragment and rMal d 1 (Biomay, lane 5), beads incubated with rBet v 1 (lane 6), Fab fragment control (lane 7) and rBet v 1 control (Biomay, lane 8). The arrow is showing rBet v 1

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