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Fig. 6 | BMC Biotechnology

Fig. 6

From: Isolation of Mal d 1 and Api g 1 - specific recombinant antibodies from mouse IgG Fab fragment libraries – Mal d 1-specific antibody exhibits cross-reactivity against Bet v 1

Fig. 6

Binding of the Fab fragment to the native allergen analysed by immunoprecipitation assay. An apple extract precipitated with MA1 Fab fragment is shown here. A silver-stained SDS-PAGE gel with low molecular weight marker (lane 1), only beads (lane 2), beads incubated with Fab fragment (lane 3), beads incubated with Fab fragment and allergen extract containing the native form of Mal d 1 (lane 4), beads incubated with Fab fragment and purified rMal d 1 (Biomay, lane 5), beads incubated with allergen extract containing the native form of Mal d 1 (lane 6), Fab fragment control (lane 7) and purified rMal d 1 control (Biomay, lane 8). The arrow is showing Mal d 1

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BMC Biotechnology

ISSN: 1472-6750

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