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Table 1 Fatty acid compositions of lipid classes from transgenic S. cerevisae

From: Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds

Construct

FA composition of PC (mol %)

C16:0

C16:1

C16:2

C18:0

C18:1

C18:2

C18:3

F2F3

5.65 ± 1.28a

46.55 ± 0.65

n.d.b

1.88 ± 0.53

43.74 ± 2.69

0.64 ± 0.03

0.96 ± 0.15

F2F3 + P1

7.98 ± 0.12

42.97 ± 0.67

4.0 ± 0.31

4.26 ± 0.12

26.78 ± 0.34

7.39 ± 0.59

6.62 ± 0.35

F2F3 + P2

7.13 ± 0.43

45.0 ± 0.36

1.52 ± 0.17

3.50 ± 0.28

34.28 ± 2.05

4.28 ± 0.45

4.26 ± 0.37

EV

4.88 ± 0.52

41.75 ± 1.51

n.d.

2.0 ± 0.26

51.35 ± 2.29

n.d.

n.d.

FA composition of DAG (mol %)

F2F3

12.17 ± 0.00

40.15 ± 0.52

n.d.

6.68 ± 0.57

40.99 ± 0.04

<0.01

<0.01

F2F3 + P1

14.54 ± 0.01

33.55 ± 1.33

n.d.

10.20 ± 0.60

28.88 ± 0.32

6.75 ± 0.33

6.06 ± 0.07

F2F3 + P2

13.35 ± 0.01

36.66 ± 0.09

n.d.

8.67 ± 0.45

34.32 ± 0.41

3.32 ± 0.12

3.68 ± 0.00

EV

10.19 ± 0.33

39.33 ± 0.53

n.d.

5.42 ± 0.23

45.05 ± 0.04

n.d.

n.d.

FA composition of TAG (mol %)

F2F3

11.99 ± 0.06

51.58 ± 0.67

n.d.

4.04 ± 0.18

32.38 ± 0.55

<0.01

<0.01

F2F3 + P1

13.0 ± 0.12

42.29 ± 0.63

2.56 ± 0.28

6.56 ± 0.11

24.09 ± 0.55

4.57 ± 0.33

6.64 ± 0.35

F2F3 + P2

12.56 ± 0.07

45.58 ± 0.54

1.66 ± 0.04

5.66 ± 0.17

26.93 ± 0.72

2.75 ± 0.08

4.85 ± 0.16

EV

10.17 ± 0.15

49.17 ± 0.82

n.d.

4.03 ± 0.13

36.63 ± 0.53

n.d.

n.d.

  1. aData represent results from three independent yeast colonies (means ± SE).
  2. bNot detected.
  3. Fatty acid composition of phosphatidylcholine (PC), diacylglycerol (DAG) and triacylglycerol (TAG) from transgenic yeast expressing LuFAD2 (F2) or LuFAD3b (F3) alone, or along with LuPDCT1 (P1) or LuPDCT2 (P2), as well as wild-type yeast transformed with empty pYESBOP vector (EV).