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Figure 3 | BMC Biotechnology

Figure 3

From: Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds

Figure 3

Analysis of PDCT activity of S. cerevisiae microsomes producing recombinant LuPDCT1 or LuPDCT2. (A) Production of radio-labeled phosphatidylcholine (PC) catalyzed by LuPDCT1 or LuPDCT2 following 15 min incubation. Radio-labeled substrate and product were separated using radio-thin layer chromatography (TLC) and visualized by phosphor-imaging. Microsomes from yeast YNL 130C cells transformed with pYES-LuPDCT1 (P1), pYES-LuPDCT2 (P2) or pYES (EV) were incubated with [14C]-glycerol- diolein in the presence of dioleoyl-PC. P1 (IN) and P2 (IN), respectively, indicate microsomes from pYES-LuPDCT1 and pYES-LuPDCT2 that were boiled prior to the assay. The phosphor image is a composite of five representative samples selected from two TLC plates which were developed under identical conditions. (B) Time course production of PC catalyzed by LuPDCT1, LuPDCT2 or EV microsomes. Data shown represent means ± SE based on three replicates.

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