Figure 1

Comparison of PDCT homologs from higher plants. (A) Alignment of deduced amino acid sequences from LuPDCT1, LuPDCT2, RcROD1 and AtROD1. Sequences were aligned using ClustalW and shading was applied using DNA Boxshade. Identical amino acids are shaded in black, while conserved substitutions are shaded in gray. Putative transmembrane domains are underlined. The five highly conserved residues in the C2 and C3 domains of the LPT family are denoted with arrows and the catalytic triads (His, His, Asp) are indicated with triangles. Protein sequences were deduced from GenBank accession numbers: Ricinus communis (RcROD1), XM_002517597; and Arabidopsis thaliana (AtROD1), At3g15820. (B) Phylogenetic tree showing the relationship between deduced PDCT proteins from different plant species. The amino acid sequences were deduced from GenBank accession numbers: Ricinus communis (RcROD1), XM_002517597; Arabidopsis thaliana (AtROD1), At3g15820; Brachypodium distachyon, XP_003563650; Glycine max, XP_003528315; Hordeum vulgare, BAK03357; Medicago truncatula, XP_003604371; Picea sitchensis, ABK25679; Populus trichocarpa, XP_002327418; Sorghum bicolor, XP_002437259; Zea maize, NP_001145186; and Oryza sativa, NP_001058029. The Brassica napus, Gossypum spp., and Helianthus annuus sequences were obtained from the computational biology and functional genomic website (http://compbio.dfci.harvard.edu/compbio) with the following gene identities: Bn, TC171107; Gs, TC240631; and Ha, TC54879. The amino acid sequences were aligned and the phylogenetic tree was constructed using Genious v. 5.3 (Biomatters Ltd. New Zealand).