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Table 1 Summary of sequencing results, quality filtering, and transcript assembly from pooled raw RNA samples vs. pooled WTA samples

From: Evaluating whole transcriptome amplification for gene profiling experiments using RNA-Seq

  Raw RNA WTA
Total sequenced reads 368,942,612 460,712,194
Filtered reads 334,297,804 395,807,422
Aligned reads 208,496,473 241,691,534
Uniquely aligned reads 173,011,711 221,886,287
Multiple aligned reads 35,484,762 19,805,247
Unmapped reads 121,884,321 152,473,973
Avg. expressed genes 15,298 15,253