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Figure 3 | BMC Biotechnology

Figure 3

From: Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans

Figure 3

ScAGU115 gene expression and protein analysis in Arabidopsis transgenic and WT plants. (A) RT-PCR analysis of ScAGU115 expression. Fragments of ScAGU115 (315 bp) and Actin2 (201 bp) were amplified from total RNA isolated from the stem tissues. (B) The soluble protein was separated on SDS-PAGE and visualized with Coomassie staining. The arrow indicates the novel protein bands that are visible on lines 4, 5, and 10. (C) The soluble proteins were immunoblotted with anti-ScAGU115 primary antibody. Control, ScAGU115 native enzyme [40].

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