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Figure 1 | BMC Biotechnology

Figure 1

From: Ligation-anchored PCR unveils immune repertoire of TCR-beta from whole blood

Figure 1

PCR method utilizing single target-specific primer to explore actively transcribed immune genes. A), The scheme of an integrative approach to explore immune repertoire from whole blood (P-U represents a universal primer sitting on the linker, whereas P-VDJ represents a TRB-specific primer targeting the consensus sequence in the constant region right after J-segment); B), DNA gel image of PCR amplified variable region (V-region) of TRB gene from a healthy donor under different conditions; C), DNA gel image of amplicons from malignant and benign patients under linker ligation or control condition.

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