Skip to main content

Table 1 Summary of the production performances for the two cell platform, Sf9 insect cells and HEK293SF mammalian cells

From: Critical assessment of influenza VLP production in Sf9 and HEK293 expression systems

 

Sucrose cushion samples

Iodixanol purified samples

Cell line

HA protein activity (HA assay)

HA protein content (SRID assay)

VLP conc. (NSTEM)

Baculovirus contamination (NSTEM)

HA protein activity (HA assay)

VLP conc. (NSTEM)

Baculovirus contamination (NSTEM)

Insect Sf9

335 HAU/ml

0.38 μg/ml

5.85 × 109 VLPs/ml

1.12 × 1011 BV/ml

32.4 HAU/ml

2.39 × 109 VLPs/ml

1.73 × 1010 BV/ml

Mammalian HEK 293

13 HAU/ml

0.092 μg/ml

1.50 × 108 VLPs/ml

3.07 × 107 BV/ml

8.11 HAU/ml

7.71 × 107 VLPs/ml

Not quantified

  1. Iodixanol fractions presented for both systems are the ones containing more VLP particles, i.e. fraction 3 for Sf9 cells (1.13 g/ml) and fraction 1 (1.16 g/ml) for HEK293SF cells. It should be noticed that for Sf9 and HEK293SF cells, sucrose cushions were concentrated respectively of 40 times and 50 times, and for iodixanol fractions of 110 times and 140 times. The values presented in this table represent the production levels (i.e. non concentrated samples).