A folded and immunogenic IgE-hyporeactive variant of the major allergen Phl p 1 produced in Escherichia coli

BMC Biotechnology

Table 1 Inhibition of binding of patients’ serum IgE (diluted 1:25) to intact Phl p 1 by polyclonal rat antibodies (diluted 1:1000) induced by immunization with the IgE-hyporeactive domain without the GST fusion tag

	*Inhibition of serum IgE binding (%)**
Patient	Rat 1	Rat 2	Rat 3	Rat 4	Mean ± SD (%)
1	83	85	84	78	83 ± 3
2	25	42	37	20	31 ± 10
3	83	74	79	71	77 ± 5
4	49	51	50	39	47 ± 6
5	67	65	51	52	59 ± 8
6	84	81	81	80	81 ± 2
7	88	85	85	87	86 ± 2
8	60	55	73	73	65 ± 9

*As compared to reactivity in the presence of sera from control rat immunized with PBS.

ISSN: 1472-6750