Figure 3From: A folded and immunogenic IgE-hyporeactive variant of the major allergen Phl p 1 produced in Escherichia coli Thermal denaturation assay using Thermofluor technology with SYPRO© dye. Unfolding fluorescent curves for the C-terminal domain of Phl p 1.0102 (red, purple) and its IgE-hyporeactive sequence variant (K8A, N11A, D55A) (blue, green). A shift of 9.7°C was observed between the IgE-hyporeactive variant and the wild-type protein.Back to article page