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Figure 2 | BMC Biotechnology

Figure 2

From: A folded and immunogenic IgE-hyporeactive variant of the major allergen Phl p 1 produced in Escherichia coli

Figure 2

Analysis of folding by CD spectroscopy. A. CD spectra of instrument ellipticity output. Spectra were buffer subtracted and nulled at 260 nm. The spectra of the C-terminal domain of Phl p 1.0102 is shown in blue and its IgE-hyporeactive (K8A, N11A, D55A) variant is shown in red. Inset zooms in on the spectra between 200–240 nm. B. CD difference spectrum of the two proteins.

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BMC Biotechnology

ISSN: 1472-6750

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