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Table 2 The composition of the media tested in this study

From: Functional expression of recombinant human trefoil factor 1 by Escherichia coli and Brevibacillus choshinensis

Medium

Composition

Carbon source

Nitrogen source

Metal ions

A

20 g/L glucose

40g/L soytone

0.012 g/L MnCl2

5 g/L yeast extract

B

20 g/L glucose,

40 g/L tryptone

0.012 g/L MnCl2

15 g/L yeast extract

C

20 g/L glucose

40 g/L soytone

0.15 g/L CaCl2

15 g/L yeast extract

D

20 g/L glucose

40 g/L tryptone

0.15 g/L CaCl2

5 g/L yeast extract

E

20 g/L soluble starch

40 g/L soytone

0.15 g/L CaCl2

5 g/L yeast extract

F

20 g/L sucrose

40 g/L soytone

0.15 g/L CaCl2;

5 g/L yeast extract

G

20 g/L glucose

40 g/L tryptone

0.15 g/L CaCl2

15 g/L yeast extract

H

50 g/L glucose

40 g/L soytone

0.15 g/L CaCl2

15 g/L yeast extract

I

20 g/L glucose

40 g/L soytone

0.15 g/L CaCl2

5 g/L yeast extract