Figure 6
The protein yields of secreted recombinant TFF1 protein by various culture media. (a) effect of metal ions and nitrogen sources; and (b) effect of carbon sources. The cultures were taken at time intervals and the OD600 were measured. The productivity of rTFF1 was estimated by comparing the density of rTFF1 bands to standard rTFF1 on SDS-PAGE gels analyzed by Gel-Pro AnalyzerTM version 3.0 (Total-Integra Technology Co., Ltd, Taipei, Taiwan).