Figure 5

The wound-healing capacities assay. (a) wound-healing capacities; and (b) statistical analysis of the wound healing travel distances. In (a), the various concentrations of rTFF1 (200 ng /mL, 400 ng/mL, 800 ng/mL) in pH 7.0 buffer and the concentration of rTFF1 (400 ng/mL) in pH 2.4 buffer were added and cultured in Ham’s F-12 K medium containing 10% FBS. The cell cultures were maintained at 37°C under an atmosphere of 5% CO₂. The wound closure was monitored and photographed at 0, 24, and 48 h with a Nikon TS100 inverted-microscope and a Nikon D5100 camera (Nikon, Japan). BC200, BC400, and BC800 represent the rTFF1 purified from B. choshinensis (pNCMO2-TFF1) with concentrations of rTFF1 200 ng/mL, 400 ng/mL, and 800 ng/mL in pH 7.0 buffer and EC200, EC400, EC800 represented the rTFF1 purified from E. coli BL21(DE3) (pET-TFF1) with concentrations of rTFF1 200 ng/mL, 400 ng/mL, and 800 ng/mL in pH 7.0 buffer. BC400 2.4 and EC400 2.4 represented rTFF1 purified from B. choshinensis (pNCMO2-TFF1) or from E. coli BL21(DE3) (pET-TFF1) with concentration of rTFF1 400 ng/mL in pH 2.4 buffer. In (b), all experiments were repeated four times and calculated with Image J 1.46r (NIH, USA). Each each bar represents the mean ± SD. NC7.0 and NC2.4 were the negative controls. The same letter represents non-significant differences of multiple comparison.