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Figure 1 | BMC Biotechnology

Figure 1

From: Functional expression of recombinant human trefoil factor 1 by Escherichia coli and Brevibacillus choshinensis

Figure 1

Expression of Escherichia coli BL21(DE3) transformants. (a) the SDS-PAGE analysis; (b) Western blot analysis; and (c) growth curve versus expression levels. In (a), The 2 × TY cultured transformants were centrifuged, and the pellet proteins were resuspended in the same volume, loaded on gel and stained with coomassie blue. In (b), the expressed supernatant proteins at various cultivation times were loaded on Tricine-SDS-PAGE, and the gel was transferred into PVDF membranes. The protein was detected with primary anti-His-Tag. (c), the transformant was cultured and the OD600 were measured at time intervals. The productivity of rTFF1 was estimated by comparing the density of rTFF1 bands to standard rTFF1 on SDS-PAGE gels analyzed by Gel-Pro AnalyzerTM version 3.0 (Total-Integra Technology Co., Ltd, Taipei, Taiwan). The arrow mark indicates the rTFF1 band.

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