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Table 3 Quantitative DNA methylation analysis

From: Improved amplification efficiency on stool samples by addition of spermidine and its use for non-invasive detection of colorectal cancer

  

Methylation (%)

CMI (%)

3rd experiment

 

NPY

PENK

WIF1

 

ID1

-

0.01 ± 0.01

0.13 ± 0.03

NA

0.14 ± 0.04

 

+

0.86 ± 0.26

1.10 ± 0.08

1.22 ± 0.41

3.18 ± 0.75

ID2

-

1.22 ± 0.21

1.31 ± 0.53

0.07 ± 0.18

2.60 ± 0.92

 

+

1.86 ± 0.11

1.35 ± 0.09

0.76 ± 0.15

3.97 ± 0.35

ID3

-

NA

NA

0.17 ± 0.07

0.17 ± 0.07

 

+

NA

0.10 ± 0.04

1.59 ± 0.22

1.69 ± 0.26

ID4

-

0.17 ± 0.08

0.32 ± 0.10

NA

0.49 ± 0.18

 

+

2.33 ± 0.32

1.88 ± 0.44

1.71 ± 0.27

5.65 ± 0.67

ID5

-

0.09 ± 0.08

0.17 ± 0.05

0.07 ± 0.01

0.33 ± 0.14

 

+

1.33 ± 0.22

1.44 ± 0.16

1.01 ± 0.07

3.78 ± 0.45

Sample (pooling

-

0.21 ± 0.06

0.27 ± 0.06

0.03 ± 0.03

0.51 ± 0.12

of five patients)

+

1.74 ± 0.30

2.10 ± 0.18

0.81 ± 0.1

4.65 ± 0.67