Sperm. | Ct | ∆Ct | AE (%) | |
---|---|---|---|---|
2nd experiment | ||||
50 ng | 0 | 28.32 ± 0.15 | 100 | |
0.5 | 25.04 ± 0.11 | −3.28 | 968 | |
1 | 25.23 ± 0.17 | −3.09 | 849 | |
5 | 28.39 ± 0.21 | 0.07 | 95 | |
100 ng | 0 | 27.04 ± 0.16 | 100 | |
0.5 | 23.74 ± 0.12 | −3.31 | 988 | |
1 | 22.97 ± 0.24 | −4.07 | 1680 | |
5 | 25.49 ± 0.17 | −1.55 | 293 | |
250 ng | 0 | NA | ND | ND |
0.5 | 28.67 ± 0.27 | ND | ND | |
1 | 23.70 ± 0.37 | ND | ND | |
5 | 23.65 ± 0.10 | ND | ND | |
500 ng | 0 | NA | ND | ND |
0.5 | NA | ND | ND | |
1 | 28.00 ± 0.56 | ND | ND | |
5 | 26.92 ± 0.30 | ND | ND |