Skip to main content

Advertisement

Table 2 Relationship between spermidine concentration and amount of stool DNA

From: Improved amplification efficiency on stool samples by addition of spermidine and its use for non-invasive detection of colorectal cancer

  Sperm. Ct ∆Ct AE (%)
2nd experiment     
50 ng 0 28.32 ± 0.15   100
  0.5 25.04 ± 0.11 −3.28 968
  1 25.23 ± 0.17 −3.09 849
  5 28.39 ± 0.21 0.07 95
100 ng 0 27.04 ± 0.16   100
  0.5 23.74 ± 0.12 −3.31 988
  1 22.97 ± 0.24 −4.07 1680
  5 25.49 ± 0.17 −1.55 293
250 ng 0 NA ND ND
  0.5 28.67 ± 0.27 ND ND
  1 23.70 ± 0.37 ND ND
  5 23.65 ± 0.10 ND ND
500 ng 0 NA ND ND
  0.5 NA ND ND
  1 28.00 ± 0.56 ND ND
  5 26.92 ± 0.30 ND ND
  1. Abbreviations: Sample, stool DNA pooled; Sperm, spermidine (mM); Ct, mean of cycle threshold value ± standard deviation value; AE, amplification efficiency; NA, non amplification; ND, not determined.