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Table 2 Relationship between spermidine concentration and amount of stool DNA

From: Improved amplification efficiency on stool samples by addition of spermidine and its use for non-invasive detection of colorectal cancer

 

Sperm.

Ct

∆Ct

AE (%)

2nd experiment

    

50 ng

0

28.32 ± 0.15

 

100

 

0.5

25.04 ± 0.11

−3.28

968

 

1

25.23 ± 0.17

−3.09

849

 

5

28.39 ± 0.21

0.07

95

100 ng

0

27.04 ± 0.16

 

100

 

0.5

23.74 ± 0.12

−3.31

988

 

1

22.97 ± 0.24

−4.07

1680

 

5

25.49 ± 0.17

−1.55

293

250 ng

0

NA

ND

ND

 

0.5

28.67 ± 0.27

ND

ND

 

1

23.70 ± 0.37

ND

ND

 

5

23.65 ± 0.10

ND

ND

500 ng

0

NA

ND

ND

 

0.5

NA

ND

ND

 

1

28.00 ± 0.56

ND

ND

 

5

26.92 ± 0.30

ND

ND

  1. Abbreviations: Sample, stool DNA pooled; Sperm, spermidine (mM); Ct, mean of cycle threshold value ± standard deviation value; AE, amplification efficiency; NA, non amplification; ND, not determined.