Figure 2

Comparison of melting curves of amplicons of the Alb gene using spermidine. We used a mixture of primers to amplify Alb gene with an amount of 50 ng of DNA templates at various spermidine concentrations ranging from 0 up to 10 mM. Melting curves of products are shown from C (A) and S (B), respectively. In (C), we highlight the melting curves of C and S in presence (C₁, S₁) and absence (C₀, S₀) of 1 mM spermidine.