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Figure 1 | BMC Biotechnology

Figure 1

From: Improved amplification efficiency on stool samples by addition of spermidine and its use for non-invasive detection of colorectal cancer

Figure 1

Verification and comparison of PCR amplification products of the albumin gene in presence and absence of spermidine. A: representative bisulfite sequencing electrophoregram of the Alb promoter using SG RT-PCR in presence of 1 mM spermidine from universal methylated human DNA (C1) and stool DNA sample (S1). All cytosine are converted to thymine noted in red resulting entirely from DNA modification. This follows after sodium bisulfite treatment (Bis) when referring to wild-type (WT) Alb gene sequence and B: the same PCR products of the Alb were analysed by agarose gel electrophoresis and revealed single amplification fragment of the predicted size (76 pb) when spermidine is present (C1, S1) and absence (C0, S0); NTC as negative control.

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