Figure 1

Verification and comparison of PCR amplification products of the albumin gene in presence and absence of spermidine. A: representative bisulfite sequencing electrophoregram of the Alb promoter using SG RT-PCR in presence of 1 mM spermidine from universal methylated human DNA (C₁) and stool DNA sample (S₁). All cytosine are converted to thymine noted in red resulting entirely from DNA modification. This follows after sodium bisulfite treatment (Bis) when referring to wild-type (WT) Alb gene sequence and B: the same PCR products of the Alb were analysed by agarose gel electrophoresis and revealed single amplification fragment of the predicted size (76 pb) when spermidine is present (C₁, S₁) and absence (C₀, S₀); NTC as negative control.