Table 2 Affinity determination by SPR
From: Structural differences of amyloid-β fibrils revealed by antibodies from phage display
K D [M] on Aβ42 antigen | |||
|---|---|---|---|
Antibody | Monomers ( X 2 ) | Protofibrils ( X 2 ) | Fibrils ( X 2 ) |
PaD97-D6 | 2.3 × 10−6 (4.9%) | 4.2 × 10−6 (2.4%) | 9.1 × 10−7 (2.3%) |
PaD172-F12 | 9.4 × 10−7 (2.4%) | 1.5 × 10−6 (1.7%) | 9.2 × 10−7 (2.4%) |
PaD213-A5 | No binding | No binding | 3.7 × 10−6 (1.1%) |
PaD233-E5 | 1.0 × 10−8 (6.6%) | 3.6 × 10−6 (1.4%) | 1.2 × 10−6 (1.4%) |
PaD236-H2 | 3.5 × 10−7 (2.5%) | 5.8 × 10−7 (1.9%) | 6.2 × 10−7 (5.7%) |