Skip to main content

Table 2 Selection and amplification efficiency of different vectors

From: Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells

Vector -HT Selection efficiency (%) 1 10 nM MTX Amplification efficiency (%) 2 50 nM MTX Amplification efficiency (%) 2
pAID 100 87 73
pAIDp 97 77 40
pAIDpp 70 40 10
pAI709Dp 0 0 0
pAI772Dp 86 90 13
pAID* 84 100 23
pAID*p 45 7 3
Untransfected control 0 0 0
  1. 1Total number of cell pools subjected to –HT selection is 96.
  2. 230 cell pools that survived –HT selection were randomly selected for sequential MTX amplification at 10 nM followed by 50 nM. Amplification efficiency is calculated as the percentage of cell pools out of the 30 initial cell pools that survived the amplification process.