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Table 2 Selection and amplification efficiency of different vectors

From: Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells

Vector

-HT Selection efficiency (%) 1

10 nM MTX Amplification efficiency (%) 2

50 nM MTX Amplification efficiency (%) 2

pAID

100

87

73

pAIDp

97

77

40

pAIDpp

70

40

10

pAI709Dp

0

0

0

pAI772Dp

86

90

13

pAID*

84

100

23

pAID*p

45

7

3

Untransfected control

0

0

0

  1. 1Total number of cell pools subjected to –HT selection is 96.
  2. 230 cell pools that survived –HT selection were randomly selected for sequential MTX amplification at 10 nM followed by 50 nM. Amplification efficiency is calculated as the percentage of cell pools out of the 30 initial cell pools that survived the amplification process.