Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells

Table 1 Sequences, amplicon sizes and sources of primer sets used for qPCR

Primer set	Primer sequences	Amplicon size (bp)	Sequence source
rhA1AT	5′-GCAATGCTACCGCAATTTTCTT-3′	72	Primer Express Software 3.0
rhA1AT	5′-CATGGGTCAGCTCGTTTTCC-3′	72	(Applied Biosystems)
dhfr	5′-ACCAGGCCACCTCAGACT-3′	120	Ng et al., 2007 [31]
dhfr	5′-GAGAGGACGCCTGGGTATT-3′	120	Ng et al., 2007 [31]
β-actin	5′-AGCTGAGAGGGAAATTGTGCG-3′	163	Fox et al., 2005 [52]
β-actin	5′-GCAACGGAACCGCTCATT-3′	163	Fox et al., 2005 [52]

rhA1AT primer set was verified by sequencing the product obtained from PCR with the first-strand cDNA from a stably transfected CHO cell line as template.

ISSN: 1472-6750