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Table 1 Generation of IL11 mutants using freeze-thawed fertilized oocytes

From: Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes

Method Vector Injected Survived Transferred Pups Analyzed Mutants
Mating Nuclease_A 76 54 (71.1) 54 4 (7.4) 4 4 (100)
Nuclease_B 72 55 (76.4) 55 5 (9.1) 5 4 (80.0)
Nickase_BC 108 94 (87.0) 94 18 (19.1) 17 1 (5.9)
FokI-dCas9_BD 55 46 (83.6) 33 6 (18.2) 6 4 (66.6)
FokI-dCas9_BD 58* 55 (94.8) 34 13 (38.2) 13 11 (84.6)
IVF Nuclease_A 32 25 (78.1) 25 3 (12.0) 3 3 (100)
Nuclease_B 34 22 (64.7) 22 2 (9.1) 2 2 (100)
Nickase_BC 32 27 (84.4) 27 6 (22.2) 6 0 (0)
FokI-dCas9_BD 39 35 (89.7) 34 6 (17.6) 6 2 (33.3)
  1. Numbers in brackets represent percentages.
  2. *Fertilized oocytes were collected from females at 8 weeks of age, whereas 5-week-old females were used for the other injections that oocytes were fertilized by mating.