Table 1 Generation of IL11 mutants using freeze-thawed fertilized oocytes
Method | Vector | Injected | Survived | Transferred | Pups | Analyzed | Mutants |
|---|---|---|---|---|---|---|---|
Mating | Nuclease_A | 76 | 54 (71.1) | 54 | 4 (7.4) | 4 | 4 (100) |
Nuclease_B | 72 | 55 (76.4) | 55 | 5 (9.1) | 5 | 4 (80.0) | |
Nickase_BC | 108 | 94 (87.0) | 94 | 18 (19.1) | 17 | 1 (5.9) | |
FokI-dCas9_BD | 55 | 46 (83.6) | 33 | 6 (18.2) | 6 | 4 (66.6) | |
FokI-dCas9_BD | 58* | 55 (94.8) | 34 | 13 (38.2) | 13 | 11 (84.6) | |
IVF | Nuclease_A | 32 | 25 (78.1) | 25 | 3 (12.0) | 3 | 3 (100) |
Nuclease_B | 34 | 22 (64.7) | 22 | 2 (9.1) | 2 | 2 (100) | |
Nickase_BC | 32 | 27 (84.4) | 27 | 6 (22.2) | 6 | 0 (0) | |
FokI-dCas9_BD | 39 | 35 (89.7) | 34 | 6 (17.6) | 6 | 2 (33.3) |