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Table 2 Resequencing results of the BAC clones

From: A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJI

  

All reads

100,000 read subset

BAC name

BAC length

Total reads

Aligned reads (%)

Ratio of coverage (%)

Average coverage depth (time)

Aligned reads

Ratio of coverage (%)

Average coverage depth (time)

MXK3

81 kb

395,498

27,585 (7.0%)

99.6%

82.3

7086

98.6%

21.3

F20D21

143 kb

558,276

86,861 (15.6%)

99.8%

149.4

15382

99.2%

26.5

F20B17

90 kb

478,300

46,409 (9.7%)

>99.9%

126.2

9173

99.9%

24.9

  1. Ratio of coverage denotes the value for specific nucleotides located on each clone. A subset of 100,000 reads was obtained using the Seqtk software.