Figure 5

Sequencing of the fungal endophyte genome with the MspJI-based DNA fragmentation method. Two independent experiments (rep1 and rep2) were performed using the single endophyte strain. (a) WGA of the endophyte genome with the Φ29 enzyme in the presence of 5-methyl-dCTP (X μM). (b) MspJI digestion of the endophyte genome-derived amplicons. (c) Flowchart of the MspJI- and physical shearing-based library prep procedures from WGA products.