Figure 4From: A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJIBAC clone sequencing based on the MspJI-based DNA fragmentation approach. (a) Amplified BAC DNA directly from glycerol stocks, using the Φ29 enzyme. In the amplification solution, 30 μM 5-methyl-dCTP were included. (b) Resequencing results of the BAC clones. Read coverage depth for each clone was visualised on the Tablet software.Back to article page