Figure 1
MspJI-enzymatic digestion of 5mC-containing PCR and Φ29 products. (a) DNA fragments amplified with the locus-specific PCR primers for the Agro_gc50 sequence under the presence of 5-methyl-dCTP (0, 2, 4 or 8 μM). (b) MspJI-digested DNA fragments derived from PCR products with each locus-specific primers and Agro gDNA as DNA template. Molar concentration denotes the 5-methyl-dCTP-concentration in PCR solution. (c) MspJI-enzymatic digestion of Φ29 enzyme-amplified DNA with randomly incorporated 5mC from a range of DNA templates. 0, 10, 15 and 20 μM denote final concentrations of 5-methyl-dCTP in the REPLI-g WGA mixture.