Figure 6From: Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thaliana Laddering of nuclear DNA from transgenic A. thaliana. DNA extracted from A. thaliana and electrophoresed for 3 h on 1.8% agarose. (A) Lane 1: DNA from non-induced plant transformed with pMDC221_yoeBGFP. (B) DNA isolated from plants transformed with pMDC221_yoeBGFP (left) and wild type plants (right) at 6 and 12 hours after induction. (C) DNA isolated from plants 24 hours after induction with 17-β-estradiol; lanes 1–6: plants transformed with pMDC221_yoeBGFP; Lanes WT1-WT3: wild type. M: 100 bp DNA ladder (Fermentas, USA); M2:1 kb DNA ladder (Fermentas, USA). Arrows indicate DNA laddering or fragmentation suggestive of apoptosis.Back to article page