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Figure 6 | BMC Biotechnology

Figure 6

From: Expression of the Streptococcus pneumoniae yoeB Chromosomal toxin gene causes Cell Death in the model plant Arabidopsis thaliana

Figure 6

Laddering of nuclear DNA from transgenic A. thaliana. DNA extracted from A. thaliana and electrophoresed for 3 h on 1.8% agarose. (A) Lane 1: DNA from non-induced plant transformed with pMDC221_yoeBGFP. (B) DNA isolated from plants transformed with pMDC221_yoeBGFP (left) and wild type plants (right) at 6 and 12 hours after induction. (C) DNA isolated from plants 24 hours after induction with 17-β-estradiol; lanes 1–6: plants transformed with pMDC221_yoeBGFP; Lanes WT1-WT3: wild type. M: 100 bp DNA ladder (Fermentas, USA); M2:1 kb DNA ladder (Fermentas, USA). Arrows indicate DNA laddering or fragmentation suggestive of apoptosis.

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