Figure 1

Vector constructs and PCR amplification from four-week-old T₂ transgenic A. thaliana using GFP-specific primers. (A) Schematic diagram of the plant inducible expression vectors that were constructed for this study based on [27]. (B) Agarose gel electrophoresis of PCR products using GFP-specific primers; samples were total DNA from plants transformed with pMDC221_yoeBGFP (lanes 1–3: transgenic line 1; lanes 4–5: transgenic line 2; lanes 6–8: transgenic line 3; lanes 9–11: transgenic line 4) or with pMDC221-GFP (lane 12) and showing the expected amplified product of 732 bp; Lane 13: sample was total DNA from a wild type Arabidopsis plant (negative control); 1 kb: 1 kb DNA ladder Marker (Fermentas).