The macronuclear and micronuclear localization of TtsfGFP-H fusion protein in
. pVTtsfGFP carrying T. thermophila cells as the control and T. thermophila carrying pVTtsfGFP-H cells were induced with 0.25 μg/ml of CdCl2 for one hour. 6 × His-TtsfGFP-H was localized to the macro- (arrowed large structure) and micronucleus (arrowed small structure). However, there was no detectable localization of the 6 × His-TtsfGFP tag protein except in the cytoplasm. The images were taken by a Leica DM6000 B fluorescence microscope equipped with a 63× objective. GFP filter was used for TtsfGFP (last column) and A filter for DAPI/Hoechst 33258 staining (middle column). The first picture columns were taken with light microscopy. The 2–3 vesicles that are seen in the cytoplasm are unknown structures.