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Table 1 Purification summary table of recombinant HP-NAP expressed in E. coli a

From: High yield purification of Helicobacter pylori neutrophil-activating protein overexpressed in Escherichia coli

Purification step Total protein (mg) b Volume (ml) Purity (%) c Amount of HP-NAP (mg) d Step recovery of HP-NAP (%) e Total recovery of HP-NAP (%) f
Whole cell lysate 26.70 20.00 69.45 18.54 100 100
Supernatant 28.60 20.00 73.34 20.98 113 113
DEAE Sephadex chromatography 19.78 59.95 95.97 18.98 90.5 102
Dialysis 18.54 57.95 97.12 18.01 94.9 97.1
Acrodisc syringe filtration 15.33 56.79 99.32 15.23 84.6 82.1
  1. aFrom 0.115 g of E.coli cell paste obtained from 50 ml culture.
  2. bProtein concentration determined by the Bradford method with bovine serum albumin (BSA) as the reference.
  3. cValues determined from densitometry measurement as described in Methods.
  4. dValues determined by multiplying the values in the column of “Total protein” and “Purity”.
  5. eValues determined by dividing the amount of HP-NAP from each purification step by that from the previous step.
  6. fValues determined by dividing the amount of HP-NAP from each purification step by that from the whole cell lysate.