Molecular and structural properties of purified recombinant HP-NAP expressed in
. The apparent molecular weight, sedimentation coefficient, and secondary structure of HP-NAP in D-PBS, pH 7.2, were analyzed by gel filtration, analytical ultracentrifugation, and CD spectroscopy, respectively, as described in Methods. A, The UV absorbance was recorded at 280 nm for the elution from a HiLoad 16/60 Superdex 200 prep grade gel gel-filtration column. The molecular weights of the protein markers were indicated at the chromatogram. B, The sedimentation coefficient distribution c(s) was shown as a function of S. The c(s) distribution was analyzed using the software program SEDFIT. C, The far UV CD spectrum of HP-NAP was recorded at the wavelength range of 195 to 260 nm. Data were representative of at least three independent experiments.