Figure 3
Optimization of the amount of proteins loaded onto resins for purifying recombinant HP-NAP from E. coli . The soluble proteins from the whole cell lysate of E. coli BL21(DE3) expressing HP-NAP were loaded onto DEAE Sephadex resins according to the indicated ratio of mg proteins per milliliter of resins to purify recombinant HP-NAP by a batch method at pH 8.0 as described in Methods. The soluble protein, indicated as load (L), the unbound fraction (A), wash fraction (B), and elution fraction (C) were analyzed by SDS-PAGE. Molecular weights (M) in kDa are indicated on the left of the stained gels. Similar results were obtained in two independent experiments.