Table 2 Hydrolytic products concentration and percentage content of beechwood xylan by rXyn11A and rXyn11A-(His) 6
From: Expression of the Thermobifida fusca xylanase Xyn11A in Pichia pastoris and its characterization
Enzyme | Reaction time (h) | Hydrolytic products concentrations (μg/mL) and percentage content | ||||
|---|---|---|---|---|---|---|
Xylose | Xylobiose | Xylotriose | Xylotetraose | xylopentaose | ||
rXyn11A | 6 | 12.0 (0.4%) | 178.6 (6.0%) | 630.8 (21.3%) | 1007.9(34.1%) | 1130.2(38.2%) |
12 | 16.9 (0.5%) | 179.7 (5.4%) | 661.5 (19.9%) | 1113.1 (33.6%) | 1347.6 (40.6%) | |
24 | 24.1 (0.7%) | 169.1 (5.2%) | 649.6 (20.0%) | 1152.6 (35.5%) | 1255.3 (38.6%) | |
rXyn11A-(His)6 | 6 | 12.5 (0.8%) | 69.8 (4.2%) | 295.3 (18.0%) | 593.1 (36.1%) | 672.2 (40.9%) |
12 | 12.8 (0.7%) | 90.2 (5.3%) | 317.8 (18.5%) | 589.0 (34.4%) | 704.0 (41.1%) | |
24 | 17.4 (1.1%) | 85.5 (5.3%) | 298.9 (18.5%) | 552.5 (34.1%) | 664.4 (41.0%) | |