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Table 1 Summary of the purification of rXyn11A and rXyn11A-(His) 6

From: Expression of the Thermobifida fusca xylanase Xyn11A in Pichia pastoris and its characterization

Purification steps

Total activity (U)

Total protein (mg)

Specific activity (U · mg −1 )

Purification (fold)

Yield (%)

P. pastoris X-33/ pPICZα-xynA

The supernatant

11576.3

77.5

149.4

1

100

Sephadex G-25

9205.5

36.1

254.9

1.7

79.5

Mono Q 5/50 GL

2462.3

6.3

390.2

2.6

21.3

Ultrafiltration

979.3

1.8

557.4

3.7

8.5

P. pastoris X-33/pPICZα-xynA-(His)6

The supernatant

9649.7

72.4

133.4

1

100

Ni-NTA agarose

3057.0

15.8

193.6

1.5

31.7

Ultrafiltration

1908.6

3.7

515.8

3.9

19.8