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Table 1 Summary of the purification of rXyn11A and rXyn11A-(His) 6

From: Expression of the Thermobifida fusca xylanase Xyn11A in Pichia pastoris and its characterization

Purification steps Total activity (U) Total protein (mg) Specific activity (U · mg −1 ) Purification (fold) Yield (%)
P. pastoris X-33/ pPICZα-xynA
The supernatant 11576.3 77.5 149.4 1 100
Sephadex G-25 9205.5 36.1 254.9 1.7 79.5
Mono Q 5/50 GL 2462.3 6.3 390.2 2.6 21.3
Ultrafiltration 979.3 1.8 557.4 3.7 8.5
P. pastoris X-33/pPICZα-xynA-(His)6
The supernatant 9649.7 72.4 133.4 1 100
Ni-NTA agarose 3057.0 15.8 193.6 1.5 31.7
Ultrafiltration 1908.6 3.7 515.8 3.9 19.8