From: Expression of the Thermobifida fusca xylanase Xyn11A in Pichia pastoris and its characterization
Purification steps | Total activity (U) | Total protein (mg) | Specific activity (U · mg −1 ) | Purification (fold) | Yield (%) |
---|---|---|---|---|---|
P. pastoris X-33/ pPICZα-xynA | |||||
The supernatant | 11576.3 | 77.5 | 149.4 | 1 | 100 |
Sephadex G-25 | 9205.5 | 36.1 | 254.9 | 1.7 | 79.5 |
Mono Q 5/50 GL | 2462.3 | 6.3 | 390.2 | 2.6 | 21.3 |
Ultrafiltration | 979.3 | 1.8 | 557.4 | 3.7 | 8.5 |
P. pastoris X-33/pPICZα-xynA-(His)6 | |||||
The supernatant | 9649.7 | 72.4 | 133.4 | 1 | 100 |
Ni-NTA agarose | 3057.0 | 15.8 | 193.6 | 1.5 | 31.7 |
Ultrafiltration | 1908.6 | 3.7 | 515.8 | 3.9 | 19.8 |