Figure 4From: Expression of the Thermobifida fusca xylanase Xyn11A in Pichia pastoris and its characterization SDS-PAGE analyses of the purification and deglycosylation of recombinant xylanases expressed in P.pastoris X-33 . Lane M: the molecular weight marker; Lane 1 and 4: supernatant of transformant harboring pPICZα-xynA-(His)6 and pPICZα-xynA, respectively; Lane 2 and 5: the purified rXyn11A-(His)6 and rXyn11A, respectively; Lane 3 and 6: the purified rXyn11A-(His)6 and rXyn11A deglycosylated with Endo H, respectively; Lane 7: Endo H.Back to article page