Figure 3
From: Expression of the Thermobifida fusca xylanase Xyn11A in Pichia pastoris and its characterization
Identification of the recombinant xylanases about 45 kDa. Lane M: the molecular weight marker; A: SDS-PAGE of recombinant xylanases stained with Coomassie Brilliant Blue R-250 (samples containing 16 μL supernatant were loaded onto a 15% polyacrylamide gel), Lane 1: supernatant of transformant harboring pPICZα-xynA-(His)6, Lane 2: supernatant of transformant harboring pPICZα-xynA; B: SDS-PAGE activity staining of recombinant xylanases using Congo Red (samples were loaded onto a 15% polyacrylamide gel), Lane 1–4: supernatants of transformant harboring pPICZα-xynA-(His)6 with different loading volumes (15 μL, 10 μL, 5 μL, 2.5 μL), Lane 5–6: supernatant of transformant harboring pPICZα-A, Lane 7–10: supernatants of transformant harboring pPICZα-xynA with different loading volumes (2.5 μL, 5 μL, 10 μL, 15 μL).