Figure 2

Modification efficiency for hairy root events. Custom-amplicon sequencing was used to measure indel frequency for each of the targeting constructs. Individual events are in orange triangles, the Cas9-tranformed control is in black circles, and average indel frequencies are vertical red bars. The miR1514 target sequence has a single mismatch to the gRNA in red. ^a n = 10, ^b n = 5, ^c n = 4.